Evaluation of Metabolic Alteration in Transgenic Rice Overexpressing Dihydroflavonol-4-reductase | Zendy

Hideyuki Takahashi | Zendy; Mitsunori Hayashi | Zendy; Fumiyuki Goto | Zendy; Shigeru Sato | Zendy; Tomoyoshi Soga | Zendy; Takaaki Nishi­oka | Zendy; Masaru Tomita | Zendy; Maki KawaiYamada | Zendy; Hirofumi Uchimiya | Zendy

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Evaluation of Metabolic Alteration in Transgenic Rice Overexpressing Dihydroflavonol-4-reductase

Author(s) -

Hideyuki Takahashi,

Mitsunori Hayashi,

Fumiyuki Goto,

Shigeru Sato,

Tomoyoshi Soga,

Takaaki Nishioka,

Masaru Tomita,

Maki KawaiYamada,

Hirofumi Uchimiya

Publication year - 2006

Publication title -

annals of botany

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.567

H-Index - 176

eISSN - 1095-8290

pISSN - 0305-7364

DOI - 10.1093/aob/mcl162

Subject(s) - biology , pentose phosphate pathway , biochemistry , glutathione reductase , glutathione , genetically modified rice , proline , citric acid cycle , amino acid , glycolysis , metabolism , enzyme , glutathione peroxidase , genetically modified crops , transgene , gene

Previous studies have shown that transgenic rice plants overexpressing YK1, which possesses dihydroflavonol-4-reductase (DFR) activity, showed biotic and abiotic stress tolerance. High throughput profiles of metabolites have also been shown in such transgenic plants by Fourier transform ion cyclotron mass spectrometry. In this study, capillary electrophoresis mass spectrometry analysis (CE/MS) was employed to identify precise metabolites such as organic acids, amino acids and sugars.

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