Flow Cytometric and Microscopic Analysis of the Effect of Tannic Acid on Plant Nuclei and Estimation of DNA Content | Zendy

João Loureiro | Zendy; Eleazar Rodriguez | Zendy; Jaroslav Doležel | Zendy; Conceição Santos | Zendy

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Flow Cytometric and Microscopic Analysis of the Effect of Tannic Acid on Plant Nuclei and Estimation of DNA Content

Author(s) -

João Loureiro,

Eleazar Rodriguez,

Jaroslav Doležel,

Conceição Santos

Publication year - 2006

Publication title -

annals of botany

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.567

H-Index - 176

eISSN - 1095-8290

pISSN - 0305-7364

DOI - 10.1093/aob/mcl140

Subject(s) - tannic acid , biology , propidium iodide , nuclear dna , dna , lysis , pisum , tris , staining , microbiology and biotechnology , lysis buffer , fluorescence , biochemistry , botany , chromatography , biophysics , genetics , mitochondrial dna , apoptosis , chemistry , programmed cell death , physics , quantum mechanics , gene

Flow cytometry (FCM) is extensively used to estimate DNA ploidy and genome size in plants. In order to determine nuclear DNA content, nuclei in suspension are stained by a DNA-specific fluorochrome and fluorescence emission is quantified. Recent studies have shown that cytosolic compounds may interfere with binding of fluorochromes to DNA, leading to flawed data. Tannic acid, a common phenolic compound, may be responsible for some of the stoichiometric errors, especially in woody plants. In this study, the effect of tannic acid on estimation of nuclear DNA content was evaluated in Pisum sativum and Zea mays, which were chosen as model species.

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