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Functional Characterization of Phosphoenolpyruvate Carboxykinase-Type C4 Leaf Anatomy: Immuno-, Cytochemical and Ultrastructural Analyses
Author(s) -
Elena V. Voznesenskaya,
Vincent R. Franceschi,
Simon D. X. Chuong,
Gerald E. Edwards
Publication year - 2006
Publication title -
annals of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.567
H-Index - 176
eISSN - 1095-8290
pISSN - 0305-7364
DOI - 10.1093/aob/mcl096
Subject(s) - biology , subfamily , phosphoenolpyruvate carboxykinase , botany , poaceae , ultrastructure , photosynthesis , phosphoenolpyruvate carboxylase , biochemistry , enzyme , gene
Species having C4 photosynthesis belonging to the phosphoenolpyruvate carboxykinase (PEP-CK) subtype, which are found only in family Poaceae, have the most complex biochemistry among the three C4 subtypes. In this study, biochemical (western blots and immunolocalization of some key photosynthetic enzymes) and structural analyses were made on several species to further understand the PEP-CK system. This included PEP-CK-type C4 species Urochloa texana (subfamily Panicoideae), Spartina alterniflora and S. anglica (subfamily Chloridoideae), and an NADP-ME-type C4 species, Echinochloa frumentacea, which has substantial levels of PEP-CK.

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