Neutral Red as a Probe for Confocal Laser Scanning Microscopy Studies of Plant Roots | Zendy

Joseph Dubrovsky | Zendy; Martin Guttenberger | Zendy; Andrés Saralegui | Zendy; Selene NapsucialyMendivil | Zendy; Boris Voigt | Zendy; František Baluška | Zendy; Diedrik Menzel | Zendy

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Neutral Red as a Probe for Confocal Laser Scanning Microscopy Studies of Plant Roots

Author(s) -

Joseph Dubrovsky,

Martin Guttenberger,

Andrés Saralegui,

Selene NapsucialyMendivil,

Boris Voigt,

František Baluška,

Diedrik Menzel

Publication year - 2006

Publication title -

annals of botany

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.567

H-Index - 176

eISSN - 1095-8290

pISSN - 0305-7364

DOI - 10.1093/aob/mcl045

Subject(s) - biology , vacuole , endodermis , biophysics , suberin , botany , cytoskeleton , arabidopsis thaliana , vital stain , plant cell , apoplast , microbiology and biotechnology , lignin , biochemistry , cell wall , staining , cytoplasm , genetics , gene , cell , mutant

Neutral red (NR), a lipophilic phenazine dye, has been widely used in various biological systems as a vital stain for bright-field microscopy. In its unprotonated form it penetrates the plasma membrane and tonoplast of viable plant cells, then due to protonation it becomes trapped in acidic compartments. The possible applications of NR for confocal laser scanning microscopy (CLSM) studies were examined in various aspects of plant root biology.

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