Plant DNA Flow Cytometry and Estimation of Nuclear Genome Size | Zendy

Jaroslav Doležel | Zendy

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Plant DNA Flow Cytometry and Estimation of Nuclear Genome Size

Author(s) -

Jaroslav Doležel

Publication year - 2004

Publication title -

annals of botany

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.567

H-Index - 176

eISSN - 1095-8290

pISSN - 0305-7364

DOI - 10.1093/aob/mci005

Subject(s) - flow cytometry , biology , dna , nuclear dna , aneuploidy , ploidy , staining , cytometry , genome size , microbiology and biotechnology , computational biology , genome , genetics , chromosome , mitochondrial dna , gene

DNA flow cytometry describes the use of flow cytometry for estimation of DNA quantity in cell nuclei. The method involves preparation of aqueous suspensions of intact nuclei whose DNA is stained using a DNA fluorochrome. The nuclei are classified according to their relative fluorescence intensity or DNA content. Because the sample preparation and analysis is convenient and rapid, DNA flow cytometry has become a popular method for ploidy screening, detection of mixoploidy and aneuploidy, cell cycle analysis, assessment of the degree of polysomaty, determination of reproductive pathway, and estimation of absolute DNA amount or genome size. While the former applications are relatively straightforward, estimation of absolute DNA amount requires special attention to possible errors in sample preparation and analysis.

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