Open AccessEfficacy Of The Bollgard Gene In Transgenic Cotton Lines Against Bollworm And Tobacco Bud-Worm, 1993
Author(s) -
J. H. Benedict,
D. R. Ring,
J. C. Corrêa,
Robert E. Buehler,
Johnson Em,
J.C. Kimball,
W. R. Deaton
Publication year - 1997
Publication title -
arthropod management tests
Language(s) - English
Resource type - Journals
eISSN - 2155-9856
pISSN - 2155-9848
DOI - 10.1093/amt/22.1.430
Subject(s) - bollworm , lint , biology , sprayer , bt cotton , horticulture , acre , agronomy , bacillus thuringiensis , botany , larva , genetics , bacteria
Efficacy Of The Bollgard Gene In Transgenic Cotton Lines Against Bollworm And Tobacco Bud-Worm, 1993 Transgenic cotton lines expressing an insecticidal protein produced by a modified crylA(c) gene from Bacillus thuringiensis kurstaki (i.e.. Bollgard Gene, Monsanto Company) were planted 17 May near Corpus Christi, Texas. Field plots were 4 row (38 inch centers) X 30 ft. Treatments (i.e., cotton lines) were arranged in a split plot RCBD and each plot replicated 6 times. The main plot was lepidopteran control (i.e., unsprayed or sprayed with Karate (0.03 lb|AIJ/acre) and Curacron (1.0 lb[AI]/acre) to control tobacco budworm, bollworm and other lepidopteran pests) and subplots were 7 cotton lines. Plots for lepidopteran suppression were sprayed on 16, 22, 29 Jul, 5, and 13 Aug with Karate and Curacron using a backpack CO2 powered sprayer and a 2-row handheld spray boom. The entire test was planted with in-furrow insecticide and oversprayed as needed for in-season control of insect pests. such as thrips, aphids, boll weevil and cotton fleahopper. Efficacy of transgenic Bollgard cottons against target lepidopteran pests was determined by ex-amining 20 flower buds and 20 bolls per plot on 14, 21, 28 Jul, 4, and 12 Aug for bollworm-tobacco budworm feeding injury, and taking lint yields/plot. Supplemental soil moisture was provided by sprinkler irrigation during the season but not within 48-h following a lepidopteran control application. The plots were hand-harvested (40 ft from middle 2 rows) and seedcotton ginned on a laboratory 10-saw gin.