Evaluation of Mycotrol Wp9504 (Beauveria Bassiana) and Provado (Imidacloprid) to Control Tarnished Plant Bug on Cotton, 1996

The efficacy of Mycotrol and Provado alone and in combination to kill TPB caged on cotton plants was studied. Cotton was planted 17 May on the UA Cotton Branch Experiment Station, Marianna, AR. Adult TPB were collected 8 Jul from flowering mustard planted in cotton. Nylon tulle (1 mm2 mesh) sleeve cages 3 ft long by 1 ft diameter were placed over the first 6 terminal nodes of flowering cotton plants in plots 50 ft long by 4 rows in width. Cages were separated across rows by 4 rows of mustard and within rows by 10 ft. TPB were placed in cages either before or after spraying (direct and indirect application). A RCB design was used with 4 blocks and 6 cages per treatment within a block (3 direct and 3 indirect applications) with 15 TPB per cage. Treatments were applied 9 Jul at 15 gpa with a 0.04% Kinetic solution as the carrier using a hand held CO2 powered boom sprayer at 35 psi with Twin Jet 8002 VS nozzles. The check was 0.04% Kinetic solution. Three, DAT, TPB were collected. Live TPB were placed in individual 30 ml plastic cups with a 2.5 cm piece of fresh green bean and monitored for mortality 3, 5, and 7 DAT. Dead TBP were placed in sporulation chambers (9 cm Petri dishes with moist filter paper) sealed with Parafilm and examined after 1 wk to assess mycoses. ANOVA and mean separation by LSD were performed using the arcsine/square root of the proportion dead from each cage.