DUPLEX POLYMERASE CHAIN REACTION WITH CONFRONTING TWO-PAIR PRIMERS (PCR-CTPP) FOR GENOTYPING ALCOHOL DEHYDROGENASE SUBUNIT (ADH2) AND ALDEHYDE DEHYDROGENASE 2 (ALDH2) | Zendy

Akiko Tamakoshi | Zendy; Nobuyuki Hamajima | Zendy; Haruya Kawase | Zendy; Kenji Wakai | Zendy; Nobuo Katsuda | Zendy; Toshiko Saito | Zendy; Hidemi Ito | Zendy; Keiko Hirose | Zendy; Toshiro Takezaki | Zendy; Keisuke Tajima | Zendy

Open Access

DUPLEX POLYMERASE CHAIN REACTION WITH CONFRONTING TWO-PAIR PRIMERS (PCR-CTPP) FOR GENOTYPING ALCOHOL DEHYDROGENASE SUBUNIT (ADH2) AND ALDEHYDE DEHYDROGENASE 2 (ALDH2)

Author(s) -

Akiko Tamakoshi,

Nobuyuki Hamajima,

Haruya Kawase,

Kenji Wakai,

Nobuo Katsuda,

Toshiko Saito,

Hidemi Ito,

Keiko Hirose,

Toshiro Takezaki,

Keisuke Tajima

Publication year - 2003

Publication title -

alcohol and alcoholism

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.747

H-Index - 99

eISSN - 1464-3502

pISSN - 0735-0414

DOI - 10.1093/alcalc/agg096

Subject(s) - genotyping , adh1b , polymerase chain reaction , aldh2 , genetics , aldehyde dehydrogenase , biology , genotype , alcohol dehydrogenase , allele , duplex (building) , polymerase , microbiology and biotechnology , enzyme , dna , dehydrogenase , gene , biochemistry , branched chain alpha keto acid dehydrogenase complex

Alcohol dehydrogenase beta subunit (ADH2) Arg47His and aldehyde dehydrogenase 2 (ALDH2) Glu487Lys were genotyped by a duplex polymerase chain reaction (PCR) with confronting two-pair primers (PCR-CTPP), which allows DNA amplification with one-tube PCR including eight primers, and subsequent electrophoresis.

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