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Alcohol dehydrogenase beta subunit (ADH2) Arg47His and aldehyde dehydrogenase 2 (ALDH2) Glu487Lys were genotyped by a duplex polymerase chain reaction (PCR) with confronting two-pair primers (PCR-CTPP), which allows DNA amplification with one-tube PCR including eight primers, and subsequent electrophoresis.

alcohol and alcoholism

DUPLEX POLYMERASE CHAIN REACTION WITH CONFRONTING TWO-PAIR PRIMERS (PCR-CTPP) FOR GENOTYPING ALCOHOL DEHYDROGENASE SUBUNIT (ADH2) AND ALDEHYDE DEHYDROGENASE 2 (ALDH2)