Dome lighting for insect imaging under a microscope | Zendy

Peter H. Kerr | Zendy; Eric M. Fisher | Zendy; Matthew L. Buffington | Zendy

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Dome lighting for insect imaging under a microscope

Author(s) -

Peter H. Kerr,

Eric M. Fisher,

Matthew L. Buffington

Publication year - 2008

Publication title -

american entomologist

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 0.364

H-Index - 41

eISSN - 2155-9902

pISSN - 1046-2821

DOI - 10.1093/ae/54.4.198

Subject(s) - microscope , dome (geology) , optics , halogen lamp , photography , light emitting diode , magnification , computer science , materials science , geology , physics , art , paleontology , visual arts

Themostbasicelementofphotographyistheinteraction oflightwiththesubjectbeingimaged.Incaseswheremagnification andclarityareofgreatimportance,suchastheimagingofinsectsfor scientificillustration,controllinglightcanbeespeciallychallenging. Theintenselightneededtorevealmicroscopicelementssuchassetae andultrastructuralsculpturingmayoftenovercompensatethelight levelsneededinotherareas,especiallyforhighcolorcontrastand/or reflectivespecimens.Projectinghalogenfiber-opticlightsoverdif- fuserssuchasMylarorStyrofoamisonemethodusedtoovercome thisimbalancesuccessfully;however,thesemethodstypicallyinvolve agreatdealofsetuptimeandexperience. Light-emittingdiodes (LEDs)areacheap,powerful,andenergy-efficientlightsourcethat isnowbeingusedmorefrequentlyintheimagingofinsectsundera microscope.Onetechniquethathasshownpromiseforquickand effectiveinsectimagingisusingLEDsinconjunctionwithanopen dometocreateadiffused,softlightarenaforphotography.Though commerciallyavailableilluminationdomesexist,creatingadome lightingsystemofyourownisinfacteasyandinexpensive.Wedetail howavarietyofdomesystemscanbemadeusingeasilyacquired materialsandidentifyafewimportantconsiderationsforeffectively imagingchallengingsubjectsunderamicroscope.

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