Screening oligonucleotide sequences for silver staining and d-galactose visual detection using RCA silver staining in a tube | Zendy

Ziwei Chen | Zendy; Xuying Duan | Zendy; Wei Hua | Zendy; Suming Tang | Zendy; Chidong Xu | Zendy; Yanlei Li | Zendy; Yifu Guan | Zendy; Guojie Zhao | Zendy

Open Access

Screening oligonucleotide sequences for silver staining and d-galactose visual detection using RCA silver staining in a tube

Author(s) -

Ziwei Chen,

Xuying Duan,

Wei Hua,

Suming Tang,

Chidong Xu,

Yanlei Li,

Yifu Guan,

Guojie Zhao

Publication year - 2018

Publication title -

acta biochimica et biophysica sinica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.771

H-Index - 57

eISSN - 1745-7270

pISSN - 1672-9145

DOI - 10.1093/abbs/gmy034

Subject(s) - staining , galactose , silver stain , rolling circle replication , oligonucleotide , microbiology and biotechnology , biology , chemistry , biochemistry , dna , genetics , polymerase

Oligonucleotides were screened for strongly silver-stained repetitive sequences. An 'AG'-clustered purine sequence showed strong staining, and the staining density can be compromised by disrupting the continuity of the 'AG'-clustered sequence. The staining-favored sequence was then employed in rolling circle amplification (RCA) for its product detection. A tube-staining method was developed for convenient and visual RCA assay. Moreover, by introducing GalR into RCA, d-galactose was detected by RCA tube-staining with naked eyes without any equipment. About 10 mM d-galactose can be easily identified, and the detection of d-galactose was specific in comparison with that of several other monosaccharides.

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