Open AccessEctopic expression of <italic>BdCIPK31</italic> confers enhanced low-temperature tolerance in transgenic tobacco plants
Author(s) -
Qingchen Luo,
Qiuhui Wei,
Ruibin Wang,
Yang Zhang,
Fan Zhang,
Yuan He,
Guangxiao Yang,
Guangyuan He
Publication year - 2017
Publication title -
acta biochimica et biophysica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.771
H-Index - 57
eISSN - 1745-7270
pISSN - 1672-9145
DOI - 10.1093/abbs/gmx140
Subject(s) - transgene , ectopic expression , microbiology and biotechnology , biology , downregulation and upregulation , gene expression , genetically modified crops , gene , biochemistry
Calcineurin B-like protein (CBL), the Ca2+ sensor, and its interacting protein kinases (CIPKs) play essential roles in plants' response to stress. However, few studies have focused on the functions of CIPKs in low-temperature response. In the present study, BdCIPK31, a cold-responsive CIPK in Brachypodium distachyon, was found to participate in low-temperature response. Ectopic expression of BdCIPK31 conferred cold tolerance in transgenic tobaccos. Further analyses indicated that expression of BdCIPK31 improved ROS detoxication and omsoprotectant biosynthesis in transgenic plants under low-temperature treatment, suggesting that the BdCIPK31 functions positively in plant adaption to the cold-induced oxidative and osmotic stresses. Moreover, BdCIPK31 could upregulate the expressions of some representative stress-related genes under cold stress. In conclusion, these findings suggest that BdCIPK31 functions positively in plant cold response.
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