TAR cloning and integrated overexpression of 6-demethylchlortetracycline biosynthetic gene cluster in &lt;italic&gt;Streptomyces aureofaciens&lt;/italic&gt; | Zendy

Naxin Wu | Zendy; He Huang | Zendy; Taoling Min | Zendy; Haifeng Hu | Zendy

Open Access

TAR cloning and integrated overexpression of 6-demethylchlortetracycline biosynthetic gene cluster in <italic>Streptomyces aureofaciens</italic>

Author(s) -

Naxin Wu,

He Huang,

Taoling Min,

Haifeng Hu

Publication year - 2017

Publication title -

acta biochimica et biophysica sinica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.771

H-Index - 57

eISSN - 1745-7270

pISSN - 1672-9145

DOI - 10.1093/abbs/gmx110

Subject(s) - streptomyces aureofaciens , gene cluster , biology , gene , cloning (programming) , gene duplication , microbiology and biotechnology , genetics , streptomyces , bacteria , computer science , programming language

6-Demethylchlortetracycline (6-DCT), a tetracycline antibiotic produced by Streptomyces aureofaciens, is a crucial precursor employed for the semi-synthesis of tigecycline, minocycline, and amadacyclin (PTK 0796). In this study, the 6-DCT biosynthetic gene cluster (BGC) was cloned from genomic DNA of a high 6-DCT-producing strain, S. aureofaciens DM-1, using the transformation-associated recombination method. An extra copy of the 6-DCT BGC was introduced and integrated into the chromosome of S. aureofaciens DM-1. Duplication of the 6-DCT BGC resulted in a maximum increase of the 6-DCT titer by 34%.

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