Galectin-1 knockdown in carcinoma-associated fibroblasts inhibits migration and invasion of human MDA-MB-231 breast cancer cells by modulating MMP-9 expression | Zendy

Xue Zhu | Zendy; Ke Wang | Zendy; Kai Zhang | Zendy; Fei Xu | Zendy; Yongxiang Yin | Zendy; Ling Zhu | Zendy; Fanfan Zhou | Zendy

Open Access

Galectin-1 knockdown in carcinoma-associated fibroblasts inhibits migration and invasion of human MDA-MB-231 breast cancer cells by modulating MMP-9 expression

Author(s) -

Xue Zhu,

Ke Wang,

Kai Zhang,

Fei Xu,

Yongxiang Yin,

Ling Zhu,

Fanfan Zhou

Publication year - 2016

Publication title -

acta biochimica et biophysica sinica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.771

H-Index - 57

eISSN - 1745-7270

pISSN - 1672-9145

DOI - 10.1093/abbs/gmw019

Subject(s) - metastasis , cancer associated fibroblasts , cancer research , gene knockdown , carcinogenesis , galectin 1 , breast cancer , matrix metalloproteinase , galectin 3 , cell migration , cancer cell , biology , cancer , tumor microenvironment , breast carcinoma , pathology , cell , chemistry , medicine , cell culture , tumor cells , genetics

Carcinoma-associated fibroblasts (CAFs) play central roles in facilitating tumor progression and metastasis in breast cancer. Galectin-1 (Gal-1), a marker of CAFs, was previously reported to be associated with tumorigenesis and metastasis of various types of tumors. The aim of this study is to investigate the role of Gal-1 in CAF-mediated breast cancer metastasis and its underlying molecular mechanisms. Our results showed that CAFs isolated from human breast tumor tissues expressed higher level of Gal-1 compared with paired normal fibroblasts, and the conditioned medium (CM) of CAFs significantly induced the migration and invasion of human MDA-MB-231 breast cancer cells. Knockdown of Gal-1 in CAFs dramatically inhibited CAF-CM-induced cell migration and invasion, probably by inhibiting the expression of matrix metalloprotein 9 (MMP-9). Our findings demonstrate that Gal-1-regulated CAFs activation promotes breast cancer cell metastasis by upregulating MMP-9 expression, which indicated that Gal-1 in CAFs might be a potential novel target for breast cancer therapy.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research