Open AccessMiR-29a/b/c regulate human circadian gene <italic>hPER1</italic> expression by targeting its 3′UTR
Author(s) -
Xueyan Zhao,
Xiaopeng Zhu,
Songbai Cheng,
Yong Xie,
Zhengrong Wang,
Yanyou Liu,
Zhou Jiang,
Jing Xiao,
Min Zhang,
Yuhui Wang
Publication year - 2014
Publication title -
acta biochimica et biophysica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.771
H-Index - 57
eISSN - 1745-7270
pISSN - 1672-9145
DOI - 10.1093/abbs/gmu007
Subject(s) - circadian rhythm , biology , gene , microbiology and biotechnology , genetics , endocrinology
Several essential biological progresses in mammals are regulated by circadian rhythms. Though the molecular mechanisms of oscillating these circadian rhythms have been uncovered, the specific functions of the circadian genes are not very clear. It has been reported that knocking down circadian genes by microRNA is a useful strategy to explore the function of the circadian rhythms. In this study, through a forward bioinformatics screening approach, we identified miR-29a/b/c as potent inhibitors for the human circadian gene hPER1. We further found that miR-29a/b/c could directly target hPER1 3'untranslated region (UTR) and down-regulate hPER1 at both mRNA and protein expression levels in human A549 cells. Thus, our findings suggested that the expression of hPER1 is regulated by miR-29a/b/c, which may also provide a new clue for the function of hPER1.