Analysis of the activity of virus internal ribosome entry site in silkworm &lt;italic&gt;Bombyx mori&lt;/italic&gt; | Zendy

Lupeng Ye | Zendy; Lanfang Zhuang | Zendy; Jisheng Li | Zendy; Zhengying You | Zendy; Jianshe Liang | Zendy; Hao Wei | Zendy; Jianrong Lin | Zendy; Boxiong Zhong | Zendy

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Analysis of the activity of virus internal ribosome entry site in silkworm <italic>Bombyx mori</italic>

Author(s) -

Lupeng Ye,

Lanfang Zhuang,

Jisheng Li,

Zhengying You,

Jianshe Liang,

Hao Wei,

Jianrong Lin,

Boxiong Zhong

Publication year - 2013

Publication title -

acta biochimica et biophysica sinica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.771

H-Index - 57

eISSN - 1745-7270

pISSN - 1672-9145

DOI - 10.1093/abbs/gmt046

Subject(s) - internal ribosome entry site , bombyx mori , sf9 , biology , spodoptera , transgene , microbiology and biotechnology , gene , virology , ribosome , rna , genetics , recombinant dna

Internal ribosome entry site (IRES) has been widely used in genetic engineering; however, the application in silkworm (Bombyx mori) has hardly been reported. In this study, the biological activity of partial sequence of Encephalomyocarditis virus (EMCV) IRES, Rhopalosiphum padi virus (RhPV) IRES, and the hybrid of IRES of EMCV and RhPV were investigated in Spodoptera frugiperda (Sf9) cell line and silkworm tissues. The hybrid IRES of EMCV and RhPV showed more effective than EMCV IRES or RhPV IRES in promoting downstream gene expression in insect and silkworm. The activities of all IRESs in middle silk gland of silkworm were higher than those in the fat body and posterior silk gland. The hybrid IRES of EMCV and RhPV was integrated into silkworm genome by transgenic technology to test biological activity of IRES. Each of the positive transgenic individuals had significant expression of report gene EGFP. These results suggested that IRES has a potential to be used in the genetic engineering research of silkworm.

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