Open AccessInvolvement of CENP-F in histone methylation
Author(s) -
Juan Du,
Yan Li,
Xueliang Zhu
Publication year - 2010
Publication title -
acta biochimica et biophysica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.771
H-Index - 57
eISSN - 1745-7270
pISSN - 1672-9145
DOI - 10.1093/abbs/gmq001
Subject(s) - epigenetics , histone , histone h3 , biology , mitosis , histone methyltransferase , kinetochore , microbiology and biotechnology , interphase , methylation , histone methylation , ezh2 , genetics , dna methylation , chromosome , gene expression , gene
CENP-F (also named mitosin) is a multifunctional protein of 350 kDa. In interphase, it is a nuclear protein, whereas in M phase it localizes to the kinetochore, the major microtubule-binding structure on chromosomes essential for chromosome segregation. CENP-F is also critical for myocyte differentiation through the interaction with Rb. It binds to ATF4 and negatively regulates the transcriptional activity of ATF4. It is also important for mitotic progression. Here we show that depletion of CENP-F by RNAi markedly downregulated the methylation of histone H3 at K4 and K9. Consistently, association of HP1a with mitotic chromosomes was largely decreased. These results uncover a novel role of CENP-F in regulation of epigenetic modification on histone H3.