Post-transcriptional regulation of &lt;italic&gt;NifA&lt;/italic&gt; expression by Hfq and RNase E complex in &lt;italic&gt;Rhizobium leguminosarum&lt;/italic&gt; bv. &lt;italic&gt;viciae&lt;/italic&gt; | Zendy

Yinghua Zhang | Zendy; Guofan Hong | Zendy

Open Access

Post-transcriptional regulation of <italic>NifA</italic> expression by Hfq and RNase E complex in <italic>Rhizobium leguminosarum</italic> bv. <italic>viciae</italic>

Author(s) -

Yinghua Zhang,

Guofan Hong

Publication year - 2009

Publication title -

acta biochimica et biophysica sinica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.771

H-Index - 57

eISSN - 1745-7270

pISSN - 1672-9145

DOI - 10.1093/abbs/gmp060

Subject(s) - rnase p , ribosomal binding site , start codon , rhizobium leguminosarum , transcriptional regulation , gene , gene expression , biology , messenger rna , microbiology and biotechnology , rna , chemistry , ribosome , genetics , bacteria , rhizobiaceae , symbiosis

NifA is the general transcriptional activator of nitrogen fixation genes in diazotrophic bacteria. In Rhizobium leguminosarum bv. viciae strain 8401/pRL1JI, the NifA gene is part of a gene cluster (fixABCXNifAB). In this study, results showed that in R. leguminosarum bv.viciae 8401/pRL1JI, host factor required (Hfq), and RNase E were involved in the post-transcriptional regulation of NifA expression. It was found that Hfq-dependent RNase E cleavage of NifA mRNA was essential for NifA translation. The cleavage site is located at 32 nucleotides upstream of the NifA translational start codon. A possible explanation based on predicted RNA secondary structure of the NifA 5'-untranslated region was that the cleavage made ribosome-binding sites accessible for translation.

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