Open AccessThe cDNA and Genomic DNA Organization of a Novel Toxin SHT-I from Spider <italic>Ornithoctonus huwena</italic>
Author(s) -
Panzhe Qiao,
Xiao-Pan Zuo,
Zhifang Chai,
Yonghua Ji
Publication year - 2004
Publication title -
acta biochimica et biophysica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.771
H-Index - 57
eISSN - 1745-7270
pISSN - 1672-9145
DOI - 10.1093/abbs/36.10.656
Subject(s) - complementary dna , biology , genomic dna , spider toxin , microbiology and biotechnology , venom , genetics , gene , biochemistry , receptor , glutamate receptor
In present study the full-length cDNA of a novel toxin from the venom gland of spider Ornithoctonus huwena, named as SHT-I, has been cloned using the strategy of rapid amplification of cDNA ends, and then the whole genomic sequence of SHT-I (Selenocosmia huwena toxin-I) was determined using sequence-specific primers synthesized based on the acquired 3′ and 5′ ends of SHT-I cDNA sequence. It is unexpectedly found that intron was lacking in the genomic sequence of SHT-I. The result might evoke an interesting question whether the gene code of inhibitor cystine-knot peptides from spider venom is distinct from that of those known toxic peptides of scorpion and cone snail.