Open AccessTime-varying mobility and turnover of actomyosin ring components during cytokinesis inSchizosaccharomyces pombe
Author(s) -
Anton Kamnev,
Saravanan Palani,
Paola Zambon,
T. H. Cheffings,
Nigel John Burroughs,
Mohan K. Balasubramanian
Publication year - 2021
Publication title -
molecular biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.463
H-Index - 225
eISSN - 1939-4586
pISSN - 1059-1524
DOI - 10.1091/mbc.e20-09-0588
Subject(s) - schizosaccharomyces pombe , cytokinesis , biology , fluorescence recovery after photobleaching , schizosaccharomyces , microbiology and biotechnology , mitosis , ring (chemistry) , yeast , fission , cell cycle , cell division , saccharomyces cerevisiae , biophysics , biochemistry , cell , physics , chemistry , organic chemistry , quantum mechanics , membrane , neutron
Cytokinesis in many eukaryotes is dependent on a contractile actomyosin ring (AMR), composed of F-actin, myosin II, and other actin and myosin II regulators. Through fluorescence recovery after photobleaching experiments, many components of the AMR have been shown to be mobile and to undergo constant exchange with the cytosolic pools. However, how the mobility of its components changes at distinct stages of mitosis and cytokinesis has not been addressed. Here, we describe the mobility of eight Schizosaccharomyces pombe AMR proteins at different stages of mitosis and cytokinesis using an approach we have developed. We identified three classes of proteins, which showed 1) high (Ain1, Myo2, Myo51), 2) low (Rng2, Mid1, Myp2, Cdc12), and 3) cell cycle-dependent (Cdc15) mobile fractions. We observed that the F-BAR protein Cdc15 undergoes a 20-30% reduction in its mobile fraction after spindle breakdown and initiation of AMR contraction. Moreover, our data indicate that this change in Cdc15 mobility is dependent on the septation initiation network (SIN). Our work offers a novel strategy for estimating cell cycle-dependent mobile protein fractions in cellular structures and provides a valuable dataset, that is of interest to researchers working on cytokinesis.