Rapid and Efficient Live Zebrafish Embryo Genotyping | Zendy

Xue Zhang | Zendy; Zhaojunjie Zhang | Zendy; Qinshun Zhao | Zendy; Xin Lou | Zendy

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Rapid and Efficient Live Zebrafish Embryo Genotyping

Author(s) -

Xue Zhang,

Zhaojunjie Zhang,

Qinshun Zhao,

Xin Lou

Publication year - 2019

Publication title -

zebrafish

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.722

H-Index - 46

eISSN - 1557-8542

pISSN - 1545-8547

DOI - 10.1089/zeb.2019.1796

Subject(s) - zebrafish , genotyping , biology , embryo , polymerase chain reaction , computational biology , genetics , genotype , gene

Most current methods for genotyping zebrafish embryos require sacrifice or raising the animal to at least 1 month of age for fin amputation. These limitations restrict the use of zebrafish and increase time and costs for experiments. This article introduces an innovative method for genotyping live zebrafish embryos. The method utilizes enzyme to extract a small amount of genetic material from the skin tissue of the embryo. Then, using conventional polymerase chain reaction (PCR) strategy, the embryo is genotyped. This approach was successful >95% of the time while maintaining high viability (>90%) of the embryo. This effective method can facilitate high-throughput screening and other applications in zebrafish.

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