Photoactivation of the CreERT2 Recombinase for Conditional Site-Specific Recombination with High Spatiotemporal Resolution | Zendy

Deepak K. Sinha | Zendy; Pierre Neveu | Zendy; Nathalie GageyEilstein | Zendy; Isabelle Aujard | Zendy; Thomas Le Saux | Zendy; Christine Rampon | Zendy; Carole Gauron | Zendy; Koichi Kawakami | Zendy; Christoph Leucht | Zendy; Laure BallyCuif | Zendy; Michel Volovitch | Zendy; David Bensimon | Zendy; Ludovic Jullien | Zendy; Sophie Vriz | Zendy

Open Access

Photoactivation of the CreER^T2 Recombinase for Conditional Site-Specific Recombination with High Spatiotemporal Resolution

Author(s) -

Deepak K. Sinha,

Pierre Neveu,

Nathalie GageyEilstein,

Isabelle Aujard,

Thomas Le Saux,

Christine Rampon,

Carole Gauron,

Koichi Kawakami,

Christoph Leucht,

Laure BallyCuif,

Michel Volovitch,

David Bensimon,

Ludovic Jullien,

Sophie Vriz

Publication year - 2010

Publication title -

zebrafish

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.722

H-Index - 46

eISSN - 1557-8542

pISSN - 1545-8547

DOI - 10.1089/zeb.2009.0632

Subject(s) - recombinase , cre recombinase , biology , zebrafish , site specific recombination , transgene , microbiology and biotechnology , cre lox recombination , recombination , reporter gene , computational biology , gene , gene expression , genetics , genetically modified mouse

We implemented a noninvasive optical method for the fast control of Cre recombinase in single cells of a live zebrafish embryo. Optical uncaging of the caged precursor of a nonendogeneous steroid by one- or two-photon illumination was used to restore Cre activity of the CreER(T2) fusion protein in specific target cells. This method labels single cells irreversibly by inducing recombination in an appropriate reporter transgenic animal and thereby can achieve high spatiotemporal resolution in the control of gene expression. This technique could be used more generally to investigate important physiological processes (e.g., in embryogenesis, organ regeneration, or carcinogenesis) with high spatiotemporal resolution (single cell and 10-min scales).

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