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Dengue Virus in Bats from Córdoba and Sucre, Colombia
Author(s) -
Alfonso Calderón,
Camilo Guzmán,
Salim Máttar,
Virginia Rodríguez,
Caty Martínez,
Lina Violet,
Jairo Martínez,
Luíz Tadeu Moraes Figueiredo
Publication year - 2019
Publication title -
vector-borne and zoonotic diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.839
H-Index - 73
eISSN - 1557-7759
pISSN - 1530-3667
DOI - 10.1089/vbz.2018.2324
Subject(s) - biology , virology , amplicon , dengue virus , dengue fever , serotype , nested polymerase chain reaction , virus , polymerase chain reaction , gene , genetics
Natural infection of dengue virus (DENV) in bats is an unexplored field in Colombia. To detect the presence of DENV in bats, a descriptive prospective study using a nonprobabilistic sampling was carried out; 286 bats in 12 sites were caught. Sample tissues of different animals were obtained; the RNA was obtained from tissues and a nested-RT-PCR was carried out and detected amplicons of 143 fragment of the NS5 gene were sequenced by the Sanger method. In nonhematophagous bats Carollia perspicillata and Phyllostomus discolor captured in Ayapel and San Carlos (Córdoba), respectively, an amplicon corresponding to NS5 was detected. The amplicons showed a high similarity with serotype-2 dengue virus (DENV-2). This is the first evidence of the DENV-2 genome in bats in from the Colombian Caribbean.

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