Reprogramming of Energy Metabolism: Increased Expression and Roles of Pyruvate Carboxylase in Papillary Thyroid Cancer | Zendy

Aurélie Strickaert | Zendy; Cyril Corbet | Zendy; Selim-Alex Spinette | Zendy; Ligia Craciun | Zendy; Geert Dom | Zendy; Guy Andry | Zendy; Denis Larsimont | Zendy; Ruddy Wattiez | Zendy; Jacques E. Dumont | Zendy; Olivier Féron | Zendy; Carine Maenhaut | Zendy

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Reprogramming of Energy Metabolism: Increased Expression and Roles of Pyruvate Carboxylase in Papillary Thyroid Cancer

Author(s) -

Aurélie Strickaert,

Cyril Corbet,

Selim-Alex Spinette,

Ligia Craciun,

Geert Dom,

Guy Andry,

Denis Larsimont,

Ruddy Wattiez,

Jacques E. Dumont,

Olivier Féron,

Carine Maenhaut

Publication year - 2019

Publication title -

thyroid

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.918

H-Index - 142

eISSN - 1557-9077

pISSN - 1050-7256

DOI - 10.1089/thy.2018.0435

Subject(s) - citric acid cycle , warburg effect , downregulation and upregulation , pyruvate carboxylase , thyroid cancer , papillary thyroid cancer , cancer cell , stromal cell , glycolysis , biology , cell cycle , cancer research , chemistry , metabolism , cancer , biochemistry , thyroid , cell , endocrinology , enzyme , genetics , gene

Background: Energy metabolism is described to be deregulated in cancer, and the Warburg effect is considered to be a major hallmark. Recently, cellular heterogeneity in tumors and the tumor microenvironment has been recognized to play an important role in several metabolic pathways in cancer. However, its contribution to papillary thyroid cancer (PTC) development and metabolism is still poorly understood. Methods: A proteomic analysis of five PTC was performed, and the cellular distribution of several upregulated metabolic proteins was investigated in the cancerous and stromal cells of these tumors. Results: Tandem mass spectrometry analysis revealed the upregulation of many metabolism-related proteins, among them pyruvate carboxylase (PC). PC knockdown in thyroid cell lines alters their proliferative and motility capacities, and measurements of oxygen consumption rates show that this enzyme is involved in the replenishment of the tricarboxylic acid cycle. Immunostainings of several upregulated metabolic proteins show that thyroid cancer cells have an increased mitochondrial oxidative metabolism compared to stromal cells. Conclusions: PTC has a very active tricarboxylic acid cycle, continuously replenished by a PC-mediated anaplerosis. This is specifically observed in the tumor cells.

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