Open AccessImage-Based, Fiber Guiding Scaffolds: A Platform for Regenerating Tissue Interfaces
Author(s) -
Chan Ho Park,
Héctor F. Ríos,
Andrei D. Taut,
Miguel PadialMolina,
Colleen L. Flanagan,
Sophia P. Pilipchuk,
Scott J. Hollister,
William V. Giannobile
Publication year - 2014
Publication title -
tissue engineering. part c methods
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.846
H-Index - 70
eISSN - 1937-3392
pISSN - 1937-3384
DOI - 10.1089/ten.tec.2013.0619
Subject(s) - scaffold , periodontal fiber , regeneration (biology) , biomedical engineering , tissue engineering , cementum , craniofacial , flexibility (engineering) , ligament , computer science , materials science , dentistry , anatomy , medicine , biology , microbiology and biotechnology , dentin , statistics , mathematics , psychiatry
In the oral and craniofacial complex, tooth loss is the most commonly acquired disfiguring injury. Among the most formidable challenges of reconstructing tooth-supporting osseous defects in the oral cavity is the regeneration of functional multi-tissue complexes involving bone, ligament, and tooth cementum. Furthermore, periodontal multi-tissue engineering with spatiotemporal orientation of the periodontal ligament (PDL) remains the most challenging obstacle for restoration of physiological loading and homeostasis. We report on the ability of a hybrid computer-designed scaffold--developed utilizing computed tomography--to predictably facilitate the regeneration and integration of dental supporting tissues. Here, we provide the protocol for rapid prototyping, manufacture, surgical implantation, and evaluation of dual-architecture scaffolds for controlling fiber orientation and facilitating morphogenesis of bone-ligament complexes. In contrast to conventional single-system methods of fibrous tissue formation, our protocol supports rigorous control of multi-compartmental scaffold architecture using computational scaffold design and manufacturing by 3D printing, as well as the evaluation of newly regenerated tissue physiology for clinical implementation.