Characteristics of High-Molecular-Weight Hyaluronic Acid as a Brain-Derived Neurotrophic Factor Scaffold in Periodontal Tissue Regeneration | Zendy

Katsuhiro Takeda | Zendy; Noriyuki Sakai | Zendy; Hideki Shiba | Zendy; Takayoshi Nagahara | Zendy; Tsuyoshi Fujita | Zendy; Mikihito Kajiya | Zendy; Tomoyuki Iwata | Zendy; Shinji Matsuda | Zendy; Kazuko Kawahara | Zendy; Hiroyuki Kawaguchi | Zendy; Hidemi Kurihara | Zendy

Open Access

Characteristics of High-Molecular-Weight Hyaluronic Acid as a Brain-Derived Neurotrophic Factor Scaffold in Periodontal Tissue Regeneration

Author(s) -

Katsuhiro Takeda,

Noriyuki Sakai,

Hideki Shiba,

Takayoshi Nagahara,

Tsuyoshi Fujita,

Mikihito Kajiya,

Tomoyuki Iwata,

Shinji Matsuda,

Kazuko Kawahara,

Hiroyuki Kawaguchi,

Hidemi Kurihara

Publication year - 2010

Publication title -

tissue engineering part a

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.964

H-Index - 111

eISSN - 1937-335X

pISSN - 1937-3341

DOI - 10.1089/ten.tea.2010.0070

Subject(s) - cementum , periodontal fiber , regeneration (biology) , hyaluronic acid , dental cementum , chemistry , neurotrophic factors , scaffold , brain derived neurotrophic factor , cementogenesis , growth factor , microbiology and biotechnology , biomedical engineering , anatomy , dentistry , biochemistry , medicine , biology , receptor , dentin

Brain-derived neurotrophic factor (BDNF), for which bovine collagen-derived atelocollagen is used as a scaffold, enhances periodontal tissue regeneration. However, a scaffold that does not contain unknown ingredients is preferable. Since the synthesized high-molecular-weight (HMW)-hyaluronic acid (HA) is safe and inexpensive, we evaluated the efficacy of HMW-HA as a BDNF scaffold. CD44, a major receptor of HA, was expressed in cultures of human periodontal ligament cells, and HMW-HA promoted the adhesion and proliferation of human periodontal ligament cells, although it did not influence the mRNA expression of bone (cementum)-related proteins. The in vitro release kinetics of BDNF from HMW-HA showed that BDNF release was sustained for 14 days. Subsequently, we examined the effect of BDNF/HMW-HA complex on periodontal tissue regeneration in dogs. A greater volume of newly formed alveolar bone and a longer newly formed cementum were observed in the BDNF/HMW-HA group than in the HMW-HA group, suggesting that HMW-HA assists the regenerative capacity of BDNF, although HMW-HA itself does not enhance periodontal tissue regeneration. Neither the poly (lactic-co-glycolic acid) group nor the BDNF/poly (lactic-co-glycolic acid) group enhanced periodontal tissue regeneration. In conclusion, HMW-HA is an adequate scaffold for the clinical application of BDNF.

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