Rapid Construction of Adeno-Associated Virus Vectors Expressing Multiple Short Hairpin RNAs with High Antiviral Activity Against Echovirus 30 | Zendy

Diana Rothe | Zendy; Gisela Wajant | Zendy; Hans-Peter Grünert | Zendy; Heinz Zeichhardt | Zendy; Henry Fechner | Zendy; Jens Kurreck | Zendy

Open Access

Rapid Construction of Adeno-Associated Virus Vectors Expressing Multiple Short Hairpin RNAs with High Antiviral Activity Against Echovirus 30

Author(s) -

Diana Rothe,

Gisela Wajant,

Hans-Peter Grünert,

Heinz Zeichhardt,

Henry Fechner,

Jens Kurreck

Publication year - 2010

Publication title -

oligonucleotides

Language(s) - English

Resource type - Journals

eISSN - 1557-8526

pISSN - 1545-4576

DOI - 10.1089/oli.2010.0236

Subject(s) - biology , small hairpin rna , virology , gene silencing , rna interference , virus , cloning (programming) , viral replication , echovirus , rna , viral interference , rna silencing , computational biology , genetics , gene , enterovirus , computer science , programming language

RNA interference has proven to be a powerful tool to inhibit viruses. For the prevention of viral escape, multiple short hairpin RNAs (shRNAs) will have to be employed. This article describes a rapid procedure for the generation of shRNA expression cassettes by parallel cloning as well as a simple strategy for the combination of selected units. After delivery of the shRNA expression cassettes with adeno-associated virus vectors, inhibition of echovirus 30 as well as silencing of an important cellular cofactor of virus replication were achieved. The procedure has the potential to be generally applicable for silencing of multiple endogenous targets or viruses.

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