Genomic Characterization of a Multidrug-Resistant and Hypermucoviscous/Hypervirulent Klebsiella quasipneumoniae subsp. similipneumoniae ST4417 Isolated from a Sewage Treatment Plant | Zendy

João Pedro Rueda Furlan | Zendy; Inara Fernanda Lage Gallo | Zendy; Tatiana Amabile de Campos | Zendy; Eliana Guedes Stehling | Zendy

Open Access

Genomic Characterization of a Multidrug-Resistant and Hypermucoviscous/Hypervirulent Klebsiella quasipneumoniae subsp. similipneumoniae ST4417 Isolated from a Sewage Treatment Plant

Author(s) -

João Pedro Rueda Furlan,

Inara Fernanda Lage Gallo,

Tatiana Amabile de Campos,

Eliana Guedes Stehling

Publication year - 2020

Publication title -

microbial drug resistance

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.889

H-Index - 70

eISSN - 1931-8448

pISSN - 1076-6294

DOI - 10.1089/mdr.2019.0417

Subject(s) - resistome , biology , multilocus sequence typing , fosfomycin , multiple drug resistance , microbiology and biotechnology , whole genome sequencing , klebsiella , virulence , antibiotic resistance , genome , genetics , gene , antimicrobial , drug resistance , genotype , antibiotics , escherichia coli , integron

Aim: Clinical strains of Klebsiella quasipneumoniae subsp. similipneumoniae have been reported worldwide. Multidrug-resistant (MDR) hypermucoviscous (hm)/hypervirulent (hv) lineages have become a global problem for public health worldwide. Therefore, this study aimed to characterize by whole-genome sequencing a MDR-hm/hv K. quasipneumoniae subsp. similipneumoniae SWT10 strain belonging to the new sequence type ST4417 isolated from a sewage treatment plant. Results: The SWT10 strain was recovered from a sewage treatment plant in Brazil and presented the hm and MDR phenotypes. Resistome analysis showed antimicrobial resistance genes associated with resistance to fluoroquinolones, β-lactams, tetracyclines, trimethoprim, aminoglycosides, sulfonamides, macrolides, and fosfomycin as well as several heavy metal resistance genes. Virulome analysis showed virulence factors related to hv lineages. Multilocus sequence typing analysis revealed the new ST4417, which was grouped in CC1584 by the minimum-spanning tree. Besides, five plasmid incompatibility groups, two prophage-related sequences, and 66 genomic islands were detected. Conclusion: This study reports for the first time the genome sequence of a MDR-hm/hv K. quasipneumoniae subsp. similipneumoniae recovered from the environment, which contributes to a better understanding about these lineages as well as for surveillance studies worldwide.

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