Open AccessDevelopment of RAP Tag, a Novel Tagging System for Protein Detection and Purification
Author(s) -
Yuki Fujii,
Mika K. Kaneko,
Satoshi Ogasawara,
Shinji Yamada,
Miyuki Yanaka,
Takuro Nakamura,
Noriko Saidoh,
Kanae Yoshida,
Ryusuke Honma,
Yukinari Kato
Publication year - 2017
Publication title -
monoclonal antibodies in immunodiagnosis and immunotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.309
H-Index - 36
ISSN - 2167-9436
DOI - 10.1089/mab.2016.0052
Subject(s) - protein tag , affinity chromatography , monoclonal antibody , tandem affinity purification , blot , microbiology and biotechnology , podoplanin , myc tag , protein purification , flow cytometry , clone (java method) , tag system , target protein , chemistry , bradford protein assay , protein g , biology , biochemistry , enzyme , antibody , recombinant dna , fusion protein , gene , computer science , immunohistochemistry , genetics , programming language , immunology
Affinity tag systems, possessing high affinity and specificity, are useful for protein detection and purification. The most suitable tag for a particular purpose should be selected from many available affinity tag systems. In this study, we developed a novel affinity tag called the "RAP tag" system, which comprises a mouse antirat podoplanin monoclonal antibody (clone PMab-2) and the RAP tag (DMVNPGLEDRIE). This system is useful not only for protein detection in Western blotting, flow cytometry, and sandwich enzyme-linked immunosorbent assay, but also for protein purification.
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