Preparation of Monoclonal Antibodies Against the Spindle Checkpoint Kinase Bub1 | Zendy

Rowena L. Lock | Zendy; Tim H. Szeto | Zendy; Alan Entwistle | Zendy; Ole Gjoerup | Zendy; Parmjit Jat | Zendy

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Preparation of Monoclonal Antibodies Against the Spindle Checkpoint Kinase Bub1

Author(s) -

Rowena L. Lock,

Tim H. Szeto,

Alan Entwistle,

Ole Gjoerup,

Parmjit Jat

Publication year - 2007

Publication title -

hybridoma

Language(s) - English

Resource type - Journals

eISSN - 1557-8348

pISSN - 1554-0014

DOI - 10.1089/hyb.2007.003

Subject(s) - bub1 , monoclonal antibody , spindle checkpoint , biology , spindle apparatus , kinetochore , microbiology and biotechnology , antibody , immunology , cell division , biochemistry , cell , gene , chromosome

The Bub1 kinase is a critical component of the spindle checkpoint involved in monitoring the separation of sister chromatids at mitosis. The viral oncoprotein Simian virus 40 large T antigen (LT) can bind and perturb the spindle checkpoint function of Bub1. We have developed three highly specific monoclonal antibodies against the Bub1 protein and have demonstrated that they can all detect Bub1 via Western blotting and immunofluorescence, in addition to their ability to immunoprecipitate Bub1.

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