Comprehensive Laboratory Evaluation of a Highly Specific Lateral Flow Assay for the Presumptive Identification ofBacillus anthracisSpores in Suspicious White Powders and Environmental Samples | Zendy

Jason G. Ramage | Zendy; Kristin Prentice | Zendy; Lindsay DePalma | Zendy; Kodumudi S. Venkateswaran | Zendy; Sruti Chivukula | Zendy; Carol Chapman | Zendy; Melissa E. Bell | Zendy; Shomik Datta | Zendy; Ajay Singh | Zendy; Alex R. Hoffmaster | Zendy; Jawad Sarwar | Zendy; Nishanth Parameswaran | Zendy; Mrinmayi Joshi | Zendy; Nagarajan Thirunavkkarasu | Zendy; V. V. Krishnan | Zendy; Stephen Morse | Zendy; Julie R. Avila | Zendy; Shashi K. Sharma | Zendy; Peter L. Estacio | Zendy; Larry H. Stanker | Zendy; David R. Hodge | Zendy; Segaran P. Pillai | Zendy

Open Access

Comprehensive Laboratory Evaluation of a Highly Specific Lateral Flow Assay for the Presumptive Identification ofBacillus anthracisSpores in Suspicious White Powders and Environmental Samples

Author(s) -

Jason G. Ramage,

Kristin Prentice,

Lindsay DePalma,

Kodumudi S. Venkateswaran,

Sruti Chivukula,

Carol Chapman,

Melissa E. Bell,

Shomik Datta,

Ajay Singh,

Alex R. Hoffmaster,

Jawad Sarwar,

Nishanth Parameswaran,

Mrinmayi Joshi,

Nagarajan Thirunavkkarasu,

V. V. Krishnan,

Stephen Morse,

Julie R. Avila,

Shashi K. Sharma,

Peter L. Estacio,

Larry H. Stanker,

David R. Hodge,

Segaran P. Pillai

Publication year - 2016

Publication title -

health security

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.705

H-Index - 37

eISSN - 2326-5108

pISSN - 2326-5094

DOI - 10.1089/hs.2016.0041

Subject(s) - bacillus anthracis , spore , microbiology and biotechnology , repeatability , immunoassay , detection limit , biology , bacillus (shape) , chromatography , chemistry , bacteria , antibody , immunology , genetics

We conducted a comprehensive, multiphase laboratory evaluation of the Anthrax BioThreat Alert(®) test strip, a lateral flow immunoassay (LFA) for the rapid detection of Bacillus anthracis spores. The study, conducted at 2 sites, evaluated this assay for the detection of spores from the Ames and Sterne strains of B. anthracis, as well as those from an additional 22 strains. Phylogenetic near neighbors, environmental background organisms, white powders, and environmental samples were also tested. The Anthrax LFA demonstrated a limit of detection of about 10(6) spores/mL (ca. 1.5 × 10(5) spores/assay). In this study, overall sensitivity of the LFA was 99.3%, and the specificity was 98.6%. The results indicated that the specificity, sensitivity, limit of detection, dynamic range, and repeatability of the assay support its use in the field for the purpose of qualitatively evaluating suspicious white powders and environmental samples for the presumptive presence of B. anthracis spores.

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