Review of a New Molecular Virus Pathotyping Method in the Context of Bioterrorism

Avian influenza virus (AIV) and Newcastle disease virus (NDV) infect various avian species including domestic poultry. Clinical manifestations vary from subclinical or mild to severe multiorgan systemic disease with a near 100% mortality rate. Severe disease is caused by highly virulent specific virus strains, termed highly pathogenic AIV and velogenic NDV. Recent controversial influenza H5 adaptation studies in ferrets have highlighted the importance of preparedness against AIV as a bioterrorism agent. Furthermore, NDV also has zoonotic potential, although symptoms in humans are mild and self-limiting for naturally occurring viruses. Thus, both of these viruses pose a direct biothreat to domestic poultry but also indirectly to humans via zoonotic transmission. For diagnosis and rapid containment of disease, it is crucial to differentiate highly pathogenic AIVs and NDVs from frequently occurring low pathogenic variants. Recently, we developed a novel strategy for pathotyping of AIV and NDV that we review here. The method should be ideal for rapid testing and surveillance in food safety, for wild bird monitoring, and for combating acts of bioterrorism.