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Cytosolic NADH-NAD(+) redox state is central to cellular metabolism and a valuable indicator of glucose and lactate metabolism in living cells. Here we sought to quantitatively determine NADH-NAD(+) redox in live cells and brain tissue using a fluorescence lifetime imaging of the genetically-encoded single-fluorophore biosensor Peredox.

Cytosolic NADH-NAD+Redox Visualized in Brain Slices by Two-Photon Fluorescence Lifetime Biosensor Imaging

Cytosolic NADH-NAD⁺Redox Visualized in Brain Slices by Two-Photon Fluorescence Lifetime Biosensor Imaging