Quantitative Redox Imaging Software | Zendy

Mark D. Fricker | Zendy

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Quantitative Redox Imaging Software

Author(s) -

Mark D. Fricker

Publication year - 2015

Publication title -

antioxidants and redox signaling

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.277

H-Index - 190

eISSN - 1557-7716

pISSN - 1523-0864

DOI - 10.1089/ars.2015.6390

Subject(s) - photobleaching , biological system , population , biophysics , fluorescence , fluorescence lifetime imaging microscopy , redox , computer science , chemistry , physics , biology , optics , organic chemistry , demography , sociology

A wealth of fluorescent reporters and imaging systems are now available to characterize dynamic physiological processes in living cells with high spatiotemporal resolution. The most reliable probes for quantitative measurements show shifts in their excitation or emission spectrum, rather than just a change in intensity, as spectral shifts are independent of optical path length, illumination intensity, probe concentration, and photobleaching, and they can be easily determined by ratiometric measurements at two wavelengths.

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