Open AccessPharmacological activity of compounds isolated from methanolic extract marine sponge xestospongia sp. against escherichia coli and staphylococcus aureus
Author(s) -
Adryan Fristiohady,
_ Wahyuni,
Baru Sadarun,
Mentarry Bafadal,
Rina Andriani,
La Ode Muhammad Julian Purnama,
Fadhliyah Malik,
Muhammad Ilyas,
Muhammad Hajrul Malaka,
Idin Sahidin
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1899/1/012048
Subject(s) - staphylococcus aureus , antibacterial activity , escherichia coli , sponge , bacteria , microbiology and biotechnology , chemistry , thin layer chromatography , agar , agar plate , column chromatography , chromatography , biology , biochemistry , botany , gene , genetics
Few previous studies show pharmacological potencies of Xestospongia sp. and have not been explored further about its antibacterial activity. Thus, this study aims to isolate and identify the isolates from Xestospongia sp. and testing their antibacterial activity. Xestospongia Sp. was macerated with methanol then isolated and was purified by using vacuum liquid chromatography (VLC), radial chromatography (RC) and thin-layer chromatography (TLC). Isolated compounds then analyzed, identified, and determined their structures using 1H-NMR Spectrophotometer and by comparising data to references and ChemDraw 8.0. Compounds obtained with various concentrations (1000; 500; 100 μg/mL) then tested against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 35218 using agar well-diffuse method. According to the study, two compounds isolated which were Saringosterol (isolate X1) and predicted-compound halenaquinone (isolate X2). The antibacterial test showed that isolate X1 was unable to inhibit bacteria‘s’ growth at each concentration, and isolate X2 showed antibacterial activity against S. aureus ATCC 25923 and E. coli ATCC 35218 at concentration 1000 and 500 μg/mL. In conclusion, 2 compounds successfully isolated from methanolic extract of Xestospongia sp. which were Saringosterol and predicted-compound halenaquninone, and only predicted-compound halenaquinone showed antibacterial activity at concentration 1000 and 500 μg/mL.