Tropical OctopusAbdopus aculeatusCan Learn to Recognize Real and Virtual Symbolic Objects

We used three consecutive operant conditioning tasks to determine whether the tropical octopus Abdopus aculeatus is able to learn to recognize a symbolic object, in either real or virtual forms. In Experiment 1, we examined whether octopuses can be conditioned to a real object (a white ball) and whether such trained individuals can select the conditioned object when they are presented with an unconditioned object. We show that octopuses learned to respond to and select the conditioned white ball in preference to the unconditioned object. In Experiment 2, we examined whether octopuses can be conditioned to an object that gradually changes from real to virtual ( i.e. , an image of that object on a computer screen). We presented four types of objects, all variations of a white ball, in a stepwise sequence as a conditioned stimulus: a real white ball, a real image of a white ball without a margin, a real image of a white ball centered within a black margin, and a virtual image of a white ball (a video on a computer screen). Individual octopuses learned to respond to all three real objects, and then a subset of these octopuses responded to the virtual object. In Experiment 3, we examined whether an octopus can learn a virtual image of an object with a specific shape not tested in Experiments 1 and 2. We presented octopuses with an image of a white cross, which was placed at various distances ( i.e. , close, medium, and far). We found that after having learned these images, octopuses could learn the virtual white cross on a computer screen. Furthermore, when we simultaneously presented octopuses with a conditioned virtual object and an unconditioned virtual object, they selected the former. Through these three experiments, we confirmed tha A. aculeatus can learn both real and virtual specific objects.