Open AccessCharacterization of Norovirus‐Associated Traveler's Diarrhea
Author(s) -
Nadim J. Ajami,
Hoonmo Koo,
Charles Darkoh,
Robert L. Atmar,
Pablo C. Okhuysen,
ZhiDong Jiang,
José Luis Hernández Flores,
Herbert L. DuPont
Publication year - 2010
Publication title -
clinical infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.44
H-Index - 336
eISSN - 1537-6591
pISSN - 1058-4838
DOI - 10.1086/653530
Subject(s) - norovirus , diarrhea , virology , phylogenetic tree , population , feces , caliciviridae , biology , outbreak , sequence analysis , phylogenetics , rotavirus , medicine , microbiology and biotechnology , veterinary medicine , virus , gene , genetics , environmental health
Traveler's diarrhea is the most common medical complaint of international visitors to developing regions. Previous findings suggested that noroviruses (NoVs) are an underappreciated cause of traveler's diarrhea. METHODS. In the present study, we sought to define the presence of NoVs in 320 acute diarrheic stool samples collected from 299 US students who traveled to Guadalajara, Cuernavaca, or Puerto Vallarta, Mexico, during the period from 2007 through 2008. Conventional and quantitative real-time polymerase chain reaction assays were used to detect and determine NoV loads in stool samples. NoV strains were characterized by purification of viral RNA followed by sequencing of the viral capsid protein 1 gene. Sequences were compared using multiple sequence alignment, and phylogenetic trees were generated to evaluate the evolutionary relatedness of the viral strains associated with cases of traveler's diarrhea.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom