Open AccessInsect Infection Model forCampylobacter jejuniReveals ThatO‐methyl Phosphoramidate Has Insecticidal Activity
Author(s) -
Olivia L. Champion,
Andrey V. Karlyshev,
Nicola J. Senior,
Martin J. Woodward,
Roberto M. La Ragione,
Sarah L. Howard,
Brendan W. Wren,
Richard W. Titball
Publication year - 2010
Publication title -
the journal of infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.69
H-Index - 252
eISSN - 1537-6613
pISSN - 0022-1899
DOI - 10.1086/650494
Subject(s) - galleria mellonella , microbiology and biotechnology , campylobacter jejuni , virulence , biology , human pathogen , virology , bacteria , gene , genetics
Galleria mellonella (wax moth) larvae have elsewhere been shown to be susceptible to pathogens such as Francisella tularensis, Burkholderia mallei, and Pseudomonas aeruginosa. We report that the larvae are rapidly killed by Campylobacter jejuni at 37C. Three strains of C. jejuni tested, 11168H (human diarrheal isolate), G1 (human Guillain-Barré syndrome isolate), and 81-176 (human diarrheal isolate), were equally effective at killing G. mellonella larvae. A panel of defined mutants of C. jejuni 11168H, in known or putative virulence genes, showed different degrees of attenuation in G. mellonella larvae. A mutant lacking the O-methyl phosphoramidate (MeOPN) capsule side group was attenuated, clearly demonstrating that MeOPN has a role in virulence. This new model of C. jejuni infection should facilitate the identification of novel virulence genes.
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