Immunological Reactivity of Blood from Healthy Humans to the rAls3p‐N Vaccine Protein | Zendy

Beverlie Baquir | Zendy; Lin Lin | Zendy; Ashraf S. Ibrahim | Zendy; Yue Fu | Zendy; Valentina Avanesian | Zendy; Ang A. Tu | Zendy; John E. Edwards | Zendy; Brad Spellberg | Zendy

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Immunological Reactivity of Blood from Healthy Humans to the rAls3p‐N Vaccine Protein

Author(s) -

Beverlie Baquir,

Lin Lin,

Ashraf S. Ibrahim,

Yue Fu,

Valentina Avanesian,

Ang A. Tu,

John E. Edwards,

Brad Spellberg

Publication year - 2009

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1086/649901

Subject(s) - antibody , immunology , immunoglobulin g , immune system , epitope , titer , recombinant dna , virology , medicine , biology , biochemistry , gene

We determined reactivity of human blood to a vaccine based on the recombinant N-terminus of candidal Als3p (rAls3p-N) in preparation for future clinical trials. Healthy donor plasma had high immunoglobulin G titers (median, 1:51,200) and lower immunoglobulin A (median, 1:3,200) and immunoglobulin E (median, 1:128) titers to rAls3p-N by enzyme-linked immunosorbent assay. rAls3p-N stimulated interferon gamma (IFN-gamma) and interleukin (IL)-17, but not IL-4, from donor lymphocytes by enzyme-linked immunosorbent spot assay and IL-12 p70, IFN-gamma, IL-17, and IL-10 by cytometric bead array. Donors reacted to diverse immunodominant epitopes. Thus, facile humoral and cellular assays can monitor immune responses to the rAls3p-N vaccine in planned clinical trials.

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