Use of Light‐Emitting Diode Fluorescence Microscopy to Detect Acid‐Fast Bacilli in Sputum | Zendy

Ben J. Marais | Zendy; Wendy Brittle | Zendy; Katrien Painczyk | Zendy; Anneke C. Hesseling | Zendy; Nulda Beyers | Zendy; E Wasserman | Zendy; Dick van Soolingen | Zendy; Robin M. Warren | Zendy

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Use of Light‐Emitting Diode Fluorescence Microscopy to Detect Acid‐Fast Bacilli in Sputum

Author(s) -

Ben J. Marais,

Wendy Brittle,

Katrien Painczyk,

Anneke C. Hesseling,

Nulda Beyers,

E Wasserman,

Dick van Soolingen,

Robin M. Warren

Publication year - 2008

Publication title -

clinical infectious diseases

Language(s) - English

Resource type - Journals

eISSN - 1537-6591

pISSN - 1058-4838

DOI - 10.1086/589248

Subject(s) - microscopy , fluorescence microscope , sputum , medicine , mycobacterium tuberculosis , optical microscope , tuberculosis , pathology , fluorescence , staining , gold standard (test) , nuclear medicine , materials science , optics , radiology , scanning electron microscope , physics , composite material

Fluorescence microscopy offers well-described benefits, compared with conventional light microscopy, for the evaluation of sputum smear samples for tuberculosis. However, its use in resource-limited settings has been limited by the high cost of the excitatory light source. We evaluated the diagnostic performance of fluorescence microscopy, using novel light-emitting diode (LED) technology as an alternative to the conventional mercury vapor lamp (MVP).

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