Diagnostic Reverse‐Transcription Polymerase Chain Reaction Kit for Filoviruses Based on the Strain Collections of all European Biosafety Level 4 Laboratories | Zendy

Marcus Panning | Zendy; Thomas M. Laue | Zendy; Stephan Ölschläger | Zendy; Markus Eickmann | Zendy; Stephan Becker | Zendy; Sabine Raith | Zendy; MarieClaude Georges Courbot | Zendy; Mikael Nilsson | Zendy; Robin Gopal | Zendy; Åke Lundkvist | Zendy; Antonino Di | Zendy; David W. Brown | Zendy; Helmut E. Meyer | Zendy; G. Lloyd | Zendy; Beate M. Kümmerer | Zendy; Stephan Günther | Zendy; Christian Drosten | Zendy

Open Access

Diagnostic Reverse‐Transcription Polymerase Chain Reaction Kit for Filoviruses Based on the Strain Collections of all European Biosafety Level 4 Laboratories

Author(s) -

Marcus Panning,

Thomas M. Laue,

Stephan Ölschläger,

Markus Eickmann,

Stephan Becker,

Sabine Raith,

MarieClaude Georges Courbot,

Mikael Nilsson,

Robin Gopal,

Åke Lundkvist,

Antonino Di,

David W. Brown,

Helmut E. Meyer,

G. Lloyd,

Beate M. Kümmerer,

Stephan Günther,

Christian Drosten

Publication year - 2007

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1086/520600

Subject(s) - ebolavirus , virology , biosafety , ebola virus , biology , polymerase chain reaction , reverse transcription polymerase chain reaction , virus , gene , genetics , microbiology and biotechnology , messenger rna

A network of European biosafety level 4 laboratories has designed the first industry-standard molecular assay for all filoviruses species, based on the strain collections of all participants. It uses 5 optimized L gene primers and 3 probes, as well as an internal control with a separate detection probe. Detection limits (probit analysis, 95% detection chance) were as follows: Zaire ebolavirus, 487 copies/mL of plasma; Sudan ebolavirus Maleo, 586 copies/mL; Sudan ebolavirus Gulu, 1128 copies/mL; Cote d'Ivoire ebolavirus, 537 copies/mL; Reston ebolavirus, 4546 copies/mL; Lake Victoria marburgvirus Musoke, 860 copies/mL; and Lake Victoria marburgvirus Ravn, 1551 copies/mL. The assay facilitates reliable detection or exclusion screening of filovirus infections.

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