Open AccessIn Vivo Expression of Interleukin‐1 Receptors during Various Experimentally Induced Inflammatory Conditions
Author(s) -
Conny J. van der Laken,
Otto C. Boerman,
Wim J.G. Oyen,
Peter Laverman,
Marjo T. P. van de Ven,
Frans H.M. Corstens,
J.W.M. van der Meer
Publication year - 1998
Publication title -
the journal of infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.69
H-Index - 252
eISSN - 1537-6613
pISSN - 0022-1899
DOI - 10.1086/517823
Subject(s) - receptor , inflammation , in vivo , interleukin 1 receptor , messenger rna , gene expression , interleukin , signal transduction , biology , interleukin 1 receptor, type i , microbiology and biotechnology , cytokine , immunology , chemistry , gene , biochemistry
Systemically administered interleukin-1 (IL-1) has been shown to preferentially bind to IL-1 receptors (IL-1Rs) in inflammation. Using radiolabeled IL-1alpha and molecular methods to assess gene expression for these receptors, the in vivo behavior of these receptors was investigated in a number of experimental inflammatory conditions. The uptake of 125I-labeled IL-1alpha in inflammatory foci significantly correlated with the mRNA expression for the type I and type II IL-1Rs (P < .05). Type II IL-1R mRNA showed a greater increase in expression than type I IL-1R mRNA. In neutropenic mice, inflammatory lesions, which are devoid of granulocytes, significantly lower 125I-labeled IL-1alpha uptake (P < .001), and type II IL-1R mRNA expression (P < .005) was found. Thus, there is strong up-regulation of IL-1Rs at sites of focal inflammation. Of interest, this mainly involved the type II IL-1R on granulocytes, which is not involved in signal transduction.
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