A Rapid Assay to Screen for Drug‐Resistant Herpes Simplex Virus | Zendy

Pablo Tebas | Zendy; David R. Scholl | Zendy; Joseph A. Jollick | Zendy; K. Mcharg | Zendy; Max Q. Arens | Zendy; Paul D. Olivo | Zendy

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A Rapid Assay to Screen for Drug‐Resistant Herpes Simplex Virus

Author(s) -

Pablo Tebas,

David R. Scholl,

Joseph A. Jollick,

K. Mcharg,

Max Q. Arens,

Paul D. Olivo

Publication year - 1998

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1086/517357

Subject(s) - herpes simplex virus , virus quantification , virology , serial dilution , virus , simplexvirus , plaque forming unit , hsl and hsv , biology , microbiology and biotechnology , herpesviridae , chemistry , medicine , viral disease , pathology , alternative medicine

A rapid assay was developed to screen for herpes simplex virus (HSV) isolates that are resistant to acyclovir and other antiviral agents. The assay is a modified plaque reduction assay (PRA) in which the number of plaques seen in the absence of acyclovir was compared with that seen in the presence of a single cutoff concentration of acyclovir (2 microg/mL). This assay utilizes a cell line that expresses beta-galactosidase only after infection with HSV. Since histochemically stained plaques are easily visualized, small plaques can be easily enumerated. This allows the assay to be performed on dilutions of untitered specimens in the small wells of a 24-well plate and allows the results to be read only 2 days after inoculation of the virus. The assay performed well compared with a standard PRA and should be a valuable tool in identifying drug-resistant HSV in a timely manner.

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