Detection of Rifampin Resistance inMycobacterium tuberculosisby Use of a Rapid, Simple, and Specific RNA/RNA Mismatch Assay | Zendy

Kevin A. Nash | Zendy; Alfred Gaytan | Zendy; Clark B. Inderlied | Zendy

Open Access

Detection of Rifampin Resistance inMycobacterium tuberculosisby Use of a Rapid, Simple, and Specific RNA/RNA Mismatch Assay

Author(s) -

Kevin A. Nash,

Alfred Gaytan,

Clark B. Inderlied

Publication year - 1997

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1086/517283

Subject(s) - mycobacterium tuberculosis , rna , biology , tuberculosis , microbiology and biotechnology , virology , dna , genetics , gene , medicine , pathology

An adaption of an RNA/RNA duplex, base pair-mismatch assay is capable of detecting rifampin resistance in Mycobacterium tuberculosis. The specificity and sensitivity of the mismatch assay in detecting rifampin resistance were 100% and 96%, respectively, when tested against 46 rifampin-resistant and rifampin-susceptible strains of M. tuberculosis. By use of a range of mycobacterial and nonmycobacterial prokaryote pathogens, the mismatch assay was shown to be specific for M. tuberculosis and Mycobacterium bovis. The assay is cost-effective compared with DNA sequencing and other molecular methods and is simple to perform and interpret. Furthermore, the assay can return a result within 24 h after receipt of an isolated organism and potentially can be used directly with smear-positive specimens.

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