Open AccessAnalysis of Conserved Ambisense Sequences within GB Virus C
Author(s) -
Yutaka Kondo,
Masashi Mizokami,
Tatsunori Nakano,
Takanobu Kato,
Yasuhito Tanaka,
Noboru Hirashima,
Ryuzo Ueda,
Mitoshi Kunimatsu,
Makoto Sasaki,
Kiyomi Yasuda,
Shiro Iino
Publication year - 1998
Publication title -
the journal of infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.69
H-Index - 252
eISSN - 1537-6613
pISSN - 0022-1899
DOI - 10.1086/515681
Subject(s) - biology , virology , gb virus c , virus , genome , nucleic acid sequence , antibody , genetics , sequence analysis , rna , flaviviridae , hepatitis c virus , gene
No analysis has been done of the ambisense of GB virus C (GBV-C). When the anti-genomes of 16 reported sequences of GBV-C were analyzed, nucleotide codons 1758 and 1402 within the anti-genome were conserved initiation and stop codons, respectively. Nucleotide sequences were also determined within the same region of 22 GBV-C strains. The anti-genomes of 38 sequences were translated and a consensus sequence was determined. In accordance with the consensus sequence, overlapping peptides were synthesized and used for the detection of anti-synthetic peptide antibodies by ELISA. The positivity of antibodies among sera with GBV-C RNA was significantly higher than among sera without GBV-C RNA (66.7% vs. 15.6%), regardless of the simultaneous presence of hepatitis B surface antigen or antibodies to hepatitis C virus (P < .05). These results indicated that a novel protein associated with GBV-C might be expressed from the ambisense of this virus.
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