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During a large pertussis outbreak, culture and polymerase chain reaction (PCR) were used to identify 149 case patients; of these case patients, 79 had positive PCR and culture results, 59 had positive PCR results and negative culture results, 11 had negative PCR results and positive culture results (10 PCR-negative, culture-positive specimens were collected &lt; or = 14 days after illness onset). PCR and culture of samples obtained &lt; or = 2 weeks after illness onset and PCR of samples obtained &gt; 2 weeks after illness onset proved to be most diagnostically useful.

clinical infectious diseases/clinical infectious diseases (online. university of chicago. press)

Evaluation of Polymerase Chain Reaction and Culture for Diagnosis of Pertussis in the Control of a County-Wide Outbreak Focused among Adolescents and Adults