Evaluation of PurifiedTaenia soliumGlycoproteins and Recombinant Antigens in the Serologic Detection of Human and Swine Cysticercosis | Zendy

Marcello Otake Sato | Zendy; Yasuhito Sako | Zendy; Minoru Nakao | Zendy; Hiroshi Yamasaki | Zendy; Kazuhiro Nakaya | Zendy; Akira Ito | Zendy

Open Access

Evaluation of PurifiedTaenia soliumGlycoproteins and Recombinant Antigens in the Serologic Detection of Human and Swine Cysticercosis

Author(s) -

Marcello Otake Sato,

Yasuhito Sako,

Minoru Nakao,

Hiroshi Yamasaki,

Kazuhiro Nakaya,

Akira Ito

Publication year - 2006

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1086/509262

Subject(s) - taenia solium , cysticercosis , virology , biology , antigen , serology , neurocysticercosis , recombinant dna , antigenicity , taenia crassiceps , taeniasis , immunology , gene , antibody , helminths , medicine , genetics , pathology , cestode infections , cestoda , zoology

Cysticercosis caused by infection with embryonated eggs of Taenia solium is an important cause of neurological disease worldwide. On the basis of mitochondrial DNA analysis, T. solium is divided into 2 (African/American and Asian) genotypes. Glycoproteins (GPs) in cyst fluid purified from the 2 genotypes of T. solium were characterized and compared with the recombinant chimeric T. solium-Ag1V1/Ag2 protein (Rec-Ag1V1/Ag2) as serodiagnostic antigens. Immunoblot analysis revealed that banding patterns of GPs differed between the 2 genotypes because of posttranslation modification, especially glycosylation. The comparison of native GPs with Rec-Ag1V1/Ag2 by enzyme-linked immunosorbent assay demonstrated that there was no statistical difference in sensitivity. In addition, the conservation of the genes encoding Ag1V1 and Ag2 in T. solium worldwide was verified. These results indicate that Rec-Ag1V1/Ag2 has great potential for usefulness in serodiagnosis as an alternative to native antigens.

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