Quantification of Viral Inactivation by Photochemical Treatment with Amotosalen and UV A Light, Using a Novel Polymerase Chain Reaction Inhibition Method with Preamplification | Zendy

JeanPierre Allain | Zendy; Jocelyn Hsu | Zendy; Manisha Pranmeth | Zendy; Deborah Hanson | Zendy; Adonis Stassinopoulos | Zendy; Lucia Fischetti | Zendy; Laurence Corash | Zendy; Lily Lin | Zendy

Open Access

Quantification of Viral Inactivation by Photochemical Treatment with Amotosalen and UV A Light, Using a Novel Polymerase Chain Reaction Inhibition Method with Preamplification

Author(s) -

JeanPierre Allain,

Jocelyn Hsu,

Manisha Pranmeth,

Deborah Hanson,

Adonis Stassinopoulos,

Lucia Fischetti,

Laurence Corash,

Lily Lin

Publication year - 2006

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1086/509260

Subject(s) - infectivity , elispot , real time polymerase chain reaction , polymerase chain reaction , microbiology and biotechnology , chemistry , virology , virus , biology , in vitro , biochemistry , gene , peripheral blood mononuclear cell

In evaluating a photochemical treatment process for inactivating parvovirus B19, there lacked simple culture methods to measure infectivity. The recently developed enzyme-linked immunospot (ELISpot) infectivity assay uses late-stage erythropoietic progenitor cells and is labor intensive and time consuming. We evaluated a novel, efficient polymerase chain reaction (PCR) inhibition assay and examined correlations with reductions in infectivity.

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