Open AccessMassTag Polymerase‐Chain‐Reaction Detection of Respiratory Pathogens, Including a New Rhinovirus Genotype, That Caused Influenza‐Like Illness in New York State during 2004–2005
Author(s) -
Daryl M. Lamson,
Neil Renwick,
Vishal Kapoor,
Zhiqiang Liu,
Gustavo Palacios,
Jingyue Ju,
Amy B. Dean,
Kirsten St. George,
Thomas Briese,
W. Ian Lipkin
Publication year - 2006
Publication title -
the journal of infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.69
H-Index - 252
eISSN - 1537-6613
pISSN - 0022-1899
DOI - 10.1086/508551
Subject(s) - rhinovirus , influenza like illness , virology , polymerase chain reaction , multiplex polymerase chain reaction , genotype , virus , biology , pathogen , clade , multiplex , orthomyxoviridae , microbiology and biotechnology , influenza a virus , gene , genetics , phylogenetics
In New York State during winter 2004, there was a high incidence of influenza-like illness that tested negative both for influenza virus, by molecular methods, and for other respiratory viruses, by virus culture. Concern that a novel pathogen might be implicated led us to implement a new multiplex diagnostic tool. MassTag polymerase chain reaction resolved 26 of 79 previously negative samples, revealing the presence of rhinoviruses in a large proportion of samples, half of which belonged to a previously uncharacterized genetic clade. In some instances, knowledge of the detected viral and/or bacterial (co)infection could have altered clinical management.
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