High Incidence of Genotypic Variance between Sequential Herpes Simplex Virus Type 2 Isolates from HIV‐1–Seropositive Patients with Recurrent Genital Herpes | Zendy

R. Wim Roest | Zendy; Jeroen Maertzdorf | Zendy; Marius Kant | Zendy; W I van der Meijden | Zendy; Albert D. M. E. Osterhaus | Zendy; Georges M. G. M. Verjans | Zendy

Open Access

High Incidence of Genotypic Variance between Sequential Herpes Simplex Virus Type 2 Isolates from HIV‐1–Seropositive Patients with Recurrent Genital Herpes

Author(s) -

R. Wim Roest,

Jeroen Maertzdorf,

Marius Kant,

W I van der Meijden,

Albert D. M. E. Osterhaus,

Georges M. G. M. Verjans

Publication year - 2006

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1086/507683

Subject(s) - genotype , virology , herpes simplex virus , genotyping , biology , superinfection , incidence (geometry) , polymerase chain reaction , virus , viral disease , herpesviridae , simplexvirus , sex organ , immunology , gene , genetics , physics , optics

We developed a polymerase chain reaction (PCR) method, based on strain-to-strain variation of DNA repeats in the herpes simplex virus type 2 (HSV-2) genes US1 and US12, to genotype HSV-2 strains and determine the incidence and risk factors associated with HSV-2 superinfection in patients with recurrent genital herpes (RGH). Forty-seven (92%) of 51 unrelated HSV-2 isolates could be distinguished. Genotyping of sequential HSV-2 isolates showed a different genotype in all of the 11 human immunodeficiency virus type 1 (HIV-1)-seropositive patients with RGH, compared with 1 of the 8 HIV-1-seronegative patients with RGH. The PCR approach developed distinguishes unrelated HSV-2 strains efficiently and indicated a high incidence of genotype variance between sequential HSV-2 isolates of HIV-1-seropositive patients with RGH.

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