Open AccessThe Granzyme B Inhibitor SERPINB9 (Protease Inhibitor 9) Circulates in Blood and Increases on Primary Cytomegalovirus Infection after Renal Transplantation
Author(s) -
Ajda T. Rowshani,
Merel C. M. Strik,
Rosalie Molenaar,
SiLa Yong,
Angela M. Wolbink,
Fréderike J. Bemelman,
C. Erik Hack,
Ineke J. M. ten Berge
Publication year - 2005
Publication title -
the journal of infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.69
H-Index - 252
eISSN - 1537-6613
pISSN - 0022-1899
DOI - 10.1086/497606
Subject(s) - granzyme b , immunology , subclinical infection , cytomegalovirus , asymptomatic , transplantation , granzyme , medicine , immune system , virology , perforin , herpesviridae , virus , cd8 , viral disease
SERPINB9 is the only known human intracellular inhibitor of granzyme B (GrB), the effector molecule in immunity against cytomegalovirus (CMV) and in renal allograft rejection. Therefore, using specific enzyme-linked immunosorbent assays, we addressed the presence of circulating SERPINB9 during primary CMV infection, subclinical rejection, acute rejection, and uncomplicated posttransplantation course. Soluble (s) SERPINB9 circulates in blood and increases on primary CMV infection. This increase was significantly higher in symptomatic than in asymptomatic patients. In contrast, sSERPINB9 levels did not change in response to subclinical or acute rejection. We demonstrated the presence of circulating sSERPINB9/sGrB complexes, which suggests that SERPINB9 has extracellular functions as well.
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